The significance of cytokines in the destruction of collagen structures in experimental periodontitis 

Aleksey N. Zakhvatov, Doctor of medical sciences, associate professor, professor of the sub-department of general surgery named after professor N.I. Atyasov, Medical Institute, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), zachvatan78@mail.ru
Dalila Ali Khaydar, Assistant of the sub-department of general and clinical dentistry, RUDN University (6 Miklukho-Maklaya street, Moscow, Russia), dhaidarA@mail.ru
Il'ya A. Zakharkin, Senior lecturer of the sub-department of dentistry, Medical Institute, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), zakharkinas@mail.ru
Tat'yana V. Tarasova, Doctor of biological sciences, professor, professor of the sub-department of normal and pathological physiology, Medical Institute, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), 9023060@mail.ru
Tat'yana V. Kurmysheva, Candidate of biological sciences, associate professor, associate professor of the sub-department of cytology, histology and embryology with courses in medical biology and molecular cell biology, Medical Institute, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), TatKurmysheva@mail.ru
Sergey A. Tambovtsev, Assistant of the sub-department of dentistry, Medical Institute, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia), 1fg922@gmail.com
Alina Yu. Parshina, Student, Medical Institute, Ogarev Mordovia State University (68 Bolshevistskaya street, Saransk, Russia),  alinaparshina2000@gmail.com

Background. Evaluation of cytokine profile indicators in dynamics and identification of their conjugation with markers of collagen metabolism in periodontitis in an experiment. Materials and methods. The experiment was performed on 62 white rats of both sexes. The animals were reproduced a model of periodontitis according to the method of the School PhD, et al. (Patent RU No. 2625295 dated 12.07.2017). The cytokine profile was assessed by the level of pro-inflammatory and anti-inflammatory cytokines, by their determination by enzyme immunoassay using a set of reagents “Bender MedSystems”. The destructive processes of connective tissue structures were determined by dynamic changes in the concentrations of the main markers of collagen metabolism: free, peptide-bound and protein-bound oxyproline, the level of which was determined by the method of N.P. Sharaev. Results. During the experimental study, when modeling periodontitis, a high level of cytokinemia was determined, which has a destructive effect on the connective tissue matrix of the periodontium, which was confirmed by an increase in the fractions of free and peptide-bound oxyproline throughout the experiment. However, by the end of the study, an increase in the level of protein-bound oxyproline was determined against the background of a continuing high level of free oxyproline, which indicated the proliferation of pathological granulations and intensification of fibrillogenesis processes. A strong correlation was determined between a high level of cytokinemia and markers of collagen metabolism, which indicates the presence of interrelations between these processes. Conclusions. The revealed violations of the physiological balance of the cytokine profile justify the inclusion in the complex therapy of periodontitis of drugs that have a pathogenetic orientation, have an immune-correcting effect, and prevent further destruction of the periodontal connective tissue matrix. Dynamic study of cytokine network parameters can be a sensitive method for detecting damage to periodontal collagen structures and a criterion for evaluating the effectiveness of treatment.

periodontitis, inflammation, cytokinemia, oxyproline, collagen destruction

Zakhvatov A.N., Khaydar D.A., Zakharkin I.A., Tarasova T.V., Kurmysheva T.V., Tambovtsev S.A., Parshina A.Yu. The significance of cytokines in the destruction of collagen structures in experimental periodontitis. Izvestiya vysshikh uchebnykh zavedeniy. Povolzhskiy region. Meditsinskie nauki = University proceedings. Volga region. Medical sciences. 2022;(4):130–139. (In Russ.). doi:10.21685/2072-3032-2023-1-12